In vitro analysis of Biofilm formation by Staphylococcus aureus and Escherichia coli isolated from Clinical samples

Abstract

Background: Biofilms are group of microorganisms which are embedded within a self-produced matrix of extracellular polymeric substance which adhere to each other. They are found to be involved in a wide range of infections in the human body like urinary tract infections (UTIs) and surgical sites infections (SSIs). Objective: This study was conducted to analysis the biofilm forming Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli) isolated from patients with UTIs and SSIs in some hospitals of Mukalla city, Hadhramout, Yemen during the period from December 2018 to May 2019. Materials and Methods: A total of 60 isolates of S. aureus and E. coli recovered from clinical samples of urine and wound swabs. In vitro biofilm formation was detected by tube method (TM), Congo red agar (CRA) method and tissue culture plate (TCP) method. Results: Biofilm formation of S. aureus and E. coli was observed in 48(80.0%), 32(53.3%) and 33(55.0%) of the isolates by TCP, TM and CRA methods respectively. Conclusion: In vitro methods showed that both S. aureus and E. coli isolated from clinical samples of UTIs and SSIs have high degree of biofilm forming ability, and the TCP method was a quantitative and reliable method for the detection of bacterial biofilm formation.